when I use V6.14.1 everything goes well, bug when I update to V6.15,the same IOC file shows LTDC RGB565 confict with usart2, when I disabled usart2, re-enabled LTDC RGB565,LTDC_R1 pin come out but no R0,R2, it must be a bug here!
LTDC_R0 is available on PE0 (used as FMC on your .ioc) and PG13 (used as GPIO_Input on your .ioc).
LTDC_R2 is available on PA1 (used as USART2_DE on your .ioc) and PC10 (used as I2C5_SDA on your .ioc). So, since you are using those pins, you can't enable LTDC_R0 and LTDC_R2.
It seems to be a regression on the STM32CubeMX V6.15.0. I've escalated internally for correction on the future (under internal ticket number 215387). Thank you so much for reporting.