Hello @Jason927 ;
As mentioned in reference manual RM0433 Rev8 section 23.3.4 QUADSPI signal interface protocol modes (Single-SPI mode): This legacy SPI mode allows just one single bit to be sent/received serially. In this mode, data are sent to the flash memory over the SO signal (whose I/O shared with IO0). Data received from the flash memory arrive via SI (whose I/O shared with IO1). The Hardware configuration for Single-SPI mode is shown in the below figure.

->AMOLED read sequence need IO0 line send and receive data
For this specially case, I think you need to configure the QUADSPI interface in Dual-SPI mode because
In dual-SPI mode, two bits are sent/received simultaneously over the IO0/IO1 signals.
So, IO0/IO1 are at high-impedance (input) during the data phase for read operations, and outputs in all other cases.
For more information about the Dual-SPI mode, I recommend you to look at RM0433 Rev8 section 23.3.4 QUADSPI signal interface protocol modes (Dual-SPI mode) and at AN4760 section 3.2.2 Dual-SPI mode.
In Dual-SPI mode the hardware configuration is similar to the one in single mode, but here two lines are used
for data, it means that data is sent and received in two lines.

I hope this help you.
Thank you.
Kaouthar